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Anti-CFLAR / FLIP Antibody (aa75-92) LS-C148270

Ordering

Wt. Vol. Conc. Price
- 50 µl - $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CFLAR / FLIP Antibodies

Anti-CFLAR / FLIP Antibody (aa191-209) IHC-plus™ LS-B3187
Rabbit Polyclonal to Human CFLAR / FLIP
Human, Mouse
IHC - Paraffin, ICC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CFLAR / FLIP Antibody (clone 2F12) IHC-plus™ LS-B5753
Mouse Monoclonal [clone 2F12] (IgG2a,k) to Human CFLAR / FLIP
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CFLAR / FLIP Antibody (aa1-480, clone NF6) LS-C344945
Mouse Monoclonal [clone NF6] (IgG1) to Human CFLAR / FLIP
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CFLAR / FLIP
Human, Monkey
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated

Details

Human CFLAR / FLIP
Rabbit
Human, Monkey (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC - Paraffin (1:1000 - 1:5000)
  • IHC - Frozen
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)

Specificity and Use

CFLAR / FLIP antibody was raised against a synthetic peptide corresponding to amino acids 75-92 (DRKAVETHLLRNPHLVS) of human FLIP, GenBank no. gi|12643547. This amino acid sequence is 100% conserved in FLIP long and short, and various FLIP isoforms as denoted in the NCBI BLAST data base. http://www. ncbi. nlm. nih. gov/blast /. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey (100%); Gibbon (94%); Marmoset (88%); Panda (82%); Mouse (76%); Rat (71%).
aa75-92
The gene encoding FLIP produces, by alternative splicing, 24 different transcripts, all with introns, putatively encoding 22 different protein isoforms. The molecular weights of these isoforms may vary. Therefore, the molecular weight of FLIP observed by western blot may vary according to the isoforms present in the sample.

Packaging

Antiserum, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About CFLAR / FLIP

O15519 NM_003879 NP_003870.4

CFLAR Antibody, C-FLIPR Antibody, C-FLIPS Antibody, C-FLIP Antibody, CASP8AP1 Antibody, Cflip Antibody, CLARP Antibody, CASH Antibody, Caspase homolog Antibody, Caspase-eight-related protein Antibody, Flice inhibitory protein Antibody, I-FLICE Antibody, Inhibitor of FLICE Antibody, FLAME Antibody, FLAME1 Antibody, MRIT Antibody, Usurpin beta Antibody, C-FLIPL Antibody, Casper Antibody, FLAME-1 Antibody, FLIP Antibody, Usurpin Antibody

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively.

Immunohistochemistry

Immunohistochemistry
IHC of FLIP in tissues cores from formalin-fixed, paraffin-embedded human brain tumor microarray using LS-C148270 at 1:2000. A-C, tissue cores from a patient with gemistocytoma. A, normal adjacent brain tissue. Astrocytes are positive for Flip expression. B, gemistocytoma (Grade II). Gemistocytes are positive for Flip expression. C, peritumoural edema brain tissue. Reactive astrocytes are positive for FLIP expression. C1, a higher magnification of C. Collectively, the data shows that astrocytes (normal), reactive astrocytes and gemistocytes are positive for Flip expression. Hematoxylin-Eos in counterstain.

Immunohistochemistry

Immunohistochemistry
IHC of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using LS-C148270 at 1:2000. A, gemistocytoma (Grade II) positive for FLIP expression. B, gemistocytoma (Grade II) negative for FLIP expression. A1 and A2 are higher magnifications from A. A, A1, and A2 show expression of FLIP in the gemistocytes of the tumor. Hematoxylin-Eosin counterstain.

Requested From: United States
Date Requested: 12/2/2016

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