Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 5D8] (IgG3) to Human CFLAR / FLIP
Western blot, ELISA
Human CFLAR / FLIP
Human (tested or 100% immunogen sequence identity)
IgG3 Monoclonal [5D8]
Protein G purified
Western blot (1:500 - 1:1000)
IHC - Paraffin
Specificity and Use
CFLAR / FLIP antibody was raised against recombinant human FLIP (1-376aa) purified from E. coli. NCBI Accession No.: NP_003870.
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500-1:1000.
PBS, pH 7.4, 0.1% sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively.
Cell lysates of mouse kidney, MCF7 and rat spleen (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FLIP (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.