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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2971-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human CFL1 / Cofilin Antibody (clone AT1C1, aa1‑166, IHC, WB) LS‑B2971
IHC‑plus™ Monoclonal Mouse anti‑Human CFL1 / Cofilin Antibody (clone AT1C1, aa1‑166, IHC, WB) LS‑B2971
Note: This antibody replaces LS-C93727
Antibody:
CFL1 / Cofilin Mouse anti-Human Monoclonal (aa1-166) (AT1C1) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CFL1 / Cofilin Mouse anti-Human Monoclonal (aa1-166) (AT1C1) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Cofilin antibody LS-B2971 is an unconjugated mouse monoclonal antibody to human Cofilin (CFL1) (aa1-166). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CFL1 / Cofilin
Synonyms
CFL1 | 18 kDa phosphoprotein | Cofilin | CFL | Cofilin 1 (non-muscle) | Cofilin, non-muscle isoform | p18
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
AT1C1
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human Cofilin 1 (1-166aa) purified from E. coli. NCBI Accession No.: NP_005498.
Epitope
aa1-166
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1:1000 - 1:2000)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2971 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2971 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CFL1 / Cofilin
Validation
Anti-CFL1 / Cofilin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CFL1 / Cofilin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CFL1 / Cofilin antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ CFL1 / Cofilin Antibody (aa1-166, clone AT1C1) for IHC, WB/Western, ELISA LS-B2971 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
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Range:
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
