Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Mouse CFB / Complement Factor B
Mouse Monoclonal (IgG1,k) to Human CFB / Complement Factor B
Western blot, ELISA
Human CFB / Complement Factor B
Human (tested or 100% immunogen sequence identity)
Protein A purified
Specificity and Use
Complement factor B isolated from human plasma.
Recognizes the Bb fragment of human factor B. Antibody binding abolishes the formation of C3bBb complexes. In Western blotting after SDS-PAGE, it reacts with the Bb fragment of factor B in non-reduced form only. After coupling to an activated CNBr-Sepharose column, this antibody is suitable for affinity purification of human factor B. Epitope specificity differs from that of LS-C22336. Also differs from LS-C22333 but slightly overlaps as determined by inhibition ELISA.
Suitable for use in ELISA and Western Blot. ELISA: 1:16000. Western Blot: Recognizes the Bb fragment of factor. B in its non-reduced form only.
PBS, pH 7.4, 15 mM sodium azide, 40% glycerol. No stabilizing proteins added.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
CFB / Complement Factor B is complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase.