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| Catalog Number | Size | Price |
|---|---|---|
| LS-B276-0.1 | 0.1 mg (2.5 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Human CENPF / CENP‑F Antibody (C‑Terminus, IHC, IF, WB) LS‑B276
IHC‑plus™ Polyclonal Rabbit anti‑Human CENPF / CENP‑F Antibody (C‑Terminus, IHC, IF, WB) LS‑B276
Note: This antibody replaces LS-C2629
Antibody:
CENPF / CENP-F Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CENPF / CENP-F Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CENP-F antibody LS-B276 is an unconjugated rabbit polyclonal antibody to human CENP-F (CENPF) (C-Terminus). Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Target
Human CENPF / CENP-F
Synonyms
CENPF | AH antigen | Centromere protein F | CENF | CENP-F | Kinetochore protein CENPF | Mitosin | CENP-F kinetochore protein | Hcp-1 | PRO1779
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A bacterial fusion protein from the C-Terminus of CENP-F.
Epitope
C-Terminus
Specificity
CENP-F.
Applications
- IHC
- IHC - Paraffin (1:500)
- Immunofluorescence (1:750)
- Western blot (1:1500)
- Immunoprecipitation (1:50)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B276 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B276 was determined to be 1:500.
Presentation
PBS, 0.05% Sodium Azide, 50% Glycerol
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CENPF / CENP-F
Validation
Anti-CENPF antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-CENPF antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ CENPF / CENP-F Antibody (C-Terminus) for IHC, IF/Immunofluorescence, WB/Western, IP LS-B276 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (3)
Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1. Bakr A, Köcher S, Volquardsen J, Petersen C, Borgmann K, Dikomey E, Rothkamm K, Mansour WY. Oncotarget. 2016 7:57679-57693. (IF; Human)
Loss of PTEN-assisted G2/M checkpoint impedes homologous recombination repair and enhances radio-curability and PARP inhibitor treatment response in prostate cancer. Mansour WY, Tennstedt P, Volquardsen J, Oing C, Kluth M, Hube-Magg C, Borgmann K, Simon R, Petersen C, Dikomey E, Rothkamm K. Scientific reports. 2018 March;8:3947. (Human)
Depletion of Akt1 and Akt2 Impairs the Repair of Radiation-Induced DNA Double Strand Breaks via Homologous Recombination. Mohammadian Gol T, Rodemann HP, Dittmann K. International journal of molecular sciences. 2019 December;20:E6316.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Species:
Human
Applications:
Flow Cytometry, Functional Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
