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Anti-CENPF / CENP-F Antibody (aa1759-2093, clone 14C10 1D8) IHC-plus™ LS-B3046

Note: This antibody replaces LS-C19955

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CENPF / CENP-F Antibodies

Anti-CENPF / CENP-F Antibody (C-Terminus) IHC-plus™ LS-B276
Rabbit Polyclonal
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-CENPF / CENP-F Antibody (aa3198-3210) IHC-plus™ LS-B2992
Goat Polyclonal to Human CENPF / CENP-F
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CENPF / CENP-F Antibody (aa1759-2093) IHC-plus™ LS-B3157
Mouse Monoclonal (IgG2a,k) to Human CENPF / CENP-F
Human
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 14C10 1D8] (IgG2a) to Human CENPF / CENP-F
Human
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

Human CENPF / CENP-F
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [14C10 1D8]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen
  • Immunofluorescence
  • Western blot (1:500)
  • Immunoprecipitation

Specificity and Use

CENPF / CENP-F antibody was raised against recombinant human CENPF.
aa1759-2093
Reacts with human Mitosin.
Immunohistochemistry: LS-B3046 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3046 was determined to be 10 ug/ml.

Packaging

PBS buffer , pH 7.2
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About CENPF / CENP-F

P49454 NM_016343 NP_057427.3

CENPF Antibody, AH antigen Antibody, Centromere protein F Antibody, CENF Antibody, CENP-F Antibody, Kinetochore protein CENPF Antibody, Mitosin Antibody, CENP-F kinetochore protein Antibody, Hcp-1 Antibody, PRO1779 Antibody

Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation.

Immunohistochemistry

Immunohistochemistry
Anti-CENPF antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3046 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-CENPF antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3046 concentration 10 ug/ml.

Requested From: United States
Date Requested: 12/8/2016

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