Target
Pig CELA1 / Pancreatic Elastase 1
Synonyms
CELA1 | El1a | Elastase I | ELA1 | Elastase 1, pancreatic | Elastase-1 | Pancreatic elastase | Pancreatic elastase I | Porcine pancreatic elastase | Pancreatic elastase 1 | Elastase
Reactivity
Pig
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Elastase [Porcine Pancreas].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas]. Cross reactivity was reported for mouse Elastase (mouse embryonic pancreas). Cross reactivity against Elastase from other tissues and species has not been determined.
Applications
- Immunofluorescence (1:100)
- Western blot (1:500 - 1:3000)
- ELISA (1:500 - 1:3000)
Usage
Suitable for immunoblotting (western or dot blot), ELISA. This product has been assayed against 1.0 ug of Elastase [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no.
LS-C60884 and ABTS as a substrate for 30 minutes at room temperature.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. Restore with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CELA1 / Pancreatic Elastase 1