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Anti-CDKN3 / KAP Antibody (clone 5H1) LS-C173327

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.13 mg/ml $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Mouse Monoclonal [clone 5H1] (IgG1) to Human CDKN3 / KAP
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human CDKN3 / KAP
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [5H1]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

CDKN3 / KAP antibody was raised against full length human recombinant protein of human CDKN3(NP_005183) produced in HEK293T cell.
Human CDKN3 / KAP

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About CDKN3 / KAP

Q16667 NM_005192 NP_005183.2

CDKN3 Antibody, CIP2 Antibody, Kinase-associated phosphatase Antibody, KAP Antibody, KAP1 Antibody, CDI1 Antibody, CDK-associated phosphatase Antibody, Kinase associated phosphatase Antibody

CDKN3 encodes a dual specificity protein phosphatase which has been identified as a cyclin-dependent kinase inhibitor and has been shown to interact with, and dephosphorylate human CDK2 kinase. CDKN3 has also been shown to associate with human CDK3 and CDC2. The protein has been reported to remove phosphate from tyrosine residues in model substrates in vitro; however, a mutant protein that bears a lesion in the putative active site cysteine has been shown to lose catalytic activity.

Immunofluorescence

Immunofluorescence
Anti-CDKN3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDKN3.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN3.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN3 antibody, and then analyzed by flow cytometry.
 

Requested From: United States
Date Requested: 12/10/2016

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