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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1347-0.05 | 0.05 mg (1 mg/ml) | $545 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human p16INK4a / CDKN2A Antibody (IHC, IF, WB) LS‑B1347
IHC‑plus™ Polyclonal Rabbit anti‑Human p16INK4a / CDKN2A Antibody (IHC, IF, WB) LS‑B1347
Note: This antibody replaces LS-C34699, LS-C56942
Antibody:
p16INK4a / CDKN2A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
p16INK4a / CDKN2A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
CDKN2A antibody LS-B1347 is an unconjugated rabbit polyclonal antibody to CDKN2A (p16INK4a) from human. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Target
Human p16INK4a / CDKN2A
Synonyms
INK4A | Multiple tumor suppressor 1 | MTS-1 | MTS1 | p16-INK4A | p16 INK4A | p16INK4A | CDKN2A | cyclin-dependent kinase inhibitor 2A
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Immunogen
An 18 amino acid synthetic peptide near the N-Terminus of human CDKN2A. The immunogen is located within the first 50 amino acids of CDKN2A.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence (20 µg/ml)
- Western blot (1 - 4 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
CDKN2A antibody can be used for detection of CDKN2A by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. Western Blot: Predicted: 17 kDa Observed: 20 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Anti-p16INK4A antibody IHC of human small intestine. Positive staining observed within the absorptive epithelium bordering the villi. Less staining observed within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-p16INK4A antibody IHC of human small intestine. Positive staining observed within the absorptive epithelium bordering the villi. Less staining observed within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-p16INK4A antibody IHC of human spleen. Positive staining observed within a subset of lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-p16INK4A antibody IHC of human spleen. Positive staining observed within a subset of lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-p16INK4A antibody IHC of human colon. Positive cytoplasmic staining within epithelial cells of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.
Anti-p16INK4A antibody IHC of human colon. Positive cytoplasmic staining within epithelial cells of the mucosa and no staining within the lamina propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.
Anti-p16INK4A antibody IHC of human kidney medulla. Positive staining observes in a subset of in tubules and no staining within vascular endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.
Anti-p16INK4A antibody IHC of human kidney medulla. Positive staining observes in a subset of in tubules and no staining within vascular endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 µg/ml.
See More About...
LSBio Ratings
IHC-plus™ p16INK4a / CDKN2A Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B1347 has an LSBio Rating of
Publications (4.3)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (4)
Tumor-promoting/progressing role of additional chromosome instability in hepatic carcinogenesis in Sgo1 (Shugoshin 1) haploinsufficient mice. Zhang Y, Reddy A, Mohammed A, Lightfoot S, Dai W, Rao CV. Carcinogenesis. Mar; (IHC, IF; Mouse)
Antagonizing pathways leading to differential dynamics in colon carcinogenesis in Shugoshin1 (Sgo1)-haploinsufficient chromosome instability model. Sanghera S, Zhang Y, Biddick L, Reddy A, Lightfoot S, Dai W, Yamada HY. Molecular carcinogenesis. 2015 Mar; (IHC; Mouse)
Role of deltaNp63(pos)CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas. Yamano S, Gi M, Tago Y, Doi K, Okada S, Hirayama Y, Tachibana H, Ishii N, Fujioka M, Tatsumi K, Wanibuchi H. Cancer science. 2016 February;107:123-32. (IHC; Mouse)
Delayed notochordal cell disappearance through integrin a5ß1 mechanotransduction during ex-vivo dynamic loading-induced intervertebral disc degeneration. Yutaro Kanda, Takashi Yurube, Yusuke Morita, Yoshiki Takeoka, Takuto Kurakawa, Ryu Tsujimoto, Kunihiko Miyazaki, Yuji Kakiuchi, Shingo Miyazaki, Zhongying Zhang, Toru Takada, Yuichi Hoshino, Koichi Masuda, Ryosuke Kuroda, Kenichiro Kakutani. Journal of orthopaedic research : official publication of the Orthopaedic Research Society. 2021 September;39:1933-1944.
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Rat
Applications:
IHC, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Reactivity:
Human
Range:
1.56-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Date Requested: 4/16/2024
