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Anti-CDKN2A / p16INK4a Antibody (aa54-75, clone 12A11) LS-C2743

Ordering

Wt. Vol. Conc. Price
- 100 µl - $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Rat Monoclonal [clone 12A11] (IgG2b) to Mouse CDKN2A / p16INK4a
Mouse
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

Mouse CDKN2A / p16INK4a
Rat
Mouse (tested or 100% immunogen sequence identity)
Human, Hamster
IgG2b Monoclonal [12A11]

Applications

Immunofluorescence, Western blot (1:500), Immunoprecipitation

Specificity and Use

CDKN2A / p16INK4a antibody was raised against a synthetic peptide containing amino acids within 1-100 of the murine p19ARF
aa54-75
murine p19ARF. It does not recognize human p14Arf nor Golden hamster p13Arf.

Packaging

Ascites, 0.1% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CDKN2A / p16INK4a

P42771 NM_000077 NP_000068.1

CDKN2A Antibody, ARF Antibody, Cdk4 inhibitor Antibody, CDK4 inhibitor p16-INK4 Antibody, INK4 Antibody, INK4A Antibody, Multiple tumor suppressor 1 Antibody, MTS-1 Antibody, MTS1 Antibody, p16-INK4 Antibody, p16-INK4A Antibody, p16INK4 Antibody, p19 Antibody, p14 Antibody, p16 Antibody, p16 INK4A Antibody, p16INK4A Antibody, p14ARF Antibody, TP16 Antibody, CDK4I Antibody, CDKN2 Antibody, CMM2 Antibody, MLM Antibody, p19ARF Antibody

Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes.

Immunofluorescence

Immunofluorescence
Immunofluorescent detection of p19Arf in wild type mouse embryo fibroblasts (MEF) at passage 5 (lanes 1, 2) and NIH3T3 cells, which have deleted the Arf gene (lanes 3, 4) were fixed in methanol/acetone and probed with LS-C2743, followed by a fluorescently labeled anti-rat IgG secondary antibody (lanes 1, 3). Cells were also stained with Hoechst dye to reveal the position of nuclei (lanes 2, 4).

Western blot

Western blot
Western blot of endogenous p19Arf using LS-C2743. Total cell lysates (25 ug) from NIH3T3 cells, which have deleted the Arf gene (lane 1) and from wild type mouse embryo fibroblasts (MEFs) at passage 6, which express p19Arf (lane 2), were resolved by SDS-PAGE. The membrane was probed with 1u g/ml purified p19Arf (LS-C2743.)

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of p19Arf using LS-C2743. Total cell lysates (250 ug) from NIH3T3 cells which have deleted the Arf gene (lane 5) and from NIH3T3 cells expressing an HA-tagged version of p19Arf (lanes 3, 4) were incubated with control rat IgG (lane 3), or LS-C2743 (lanes 4, 5) and protein G- Sepharose. Precipitated proteins were resolved by SDS-PAGE and analyzed by Western blotting using an anti-HA antibody. Unprecipitated total cell lysates (25 ug, equivalent to 10% of immunoprecipitation) f.
 

Requested From: United States
Date Requested: 9/28/2016

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