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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDKN1C / p57 Kip2 Antibody (aa34‑64) LS‑C168622
P57 Kip2 antibody LS-C168622 is an unconjugated rabbit polyclonal antibody to p57 Kip2 (CDKN1C) from human and mouse. Validated for IF and WB.
Catalog
Size
Price
LS-C168622-400
400 µl
Unavailable

Popular CDKN1C / p57 Kip2 Products

Formalin-fixed, paraffin-embedded human colon carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Formalin-fixed, paraffin-embedded human colon cancer stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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Product Description

P57 Kip2 antibody LS-C168622 is an unconjugated rabbit polyclonal antibody to p57 Kip2 (CDKN1C) from human and mouse. Validated for IF and WB.
About CDKN1C / p57 Kip2
Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life. P49918 nm_000076 NP_000067.1

CDKN1C Antibody, BWS Antibody, KIP2 Antibody, IMAGE Antibody, p57Kip2 Antibody, p57 Antibody, p57 KIP2 Antibody, Beckwith-Wiedemann syndrome Antibody, WBS Antibody, BWCR Antibody


Specifications

Target
Human CDKN1C / p57 Kip2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Blocking Peptide
LS-E613 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C168622. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa34-64
Specificity
This CDKN1C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-64 amino acids from the N-terminal region of human CDKN1C.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Fluorescent image of A549 cell stained with CDKN1C Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1C primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). CDKN1C immunoreactivity is localized to Nucleus significantly.
Fluorescent image of A549 cell stained with CDKN1C Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1C primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). CDKN1C immunoreactivity is localized to Nucleus significantly.

Western blot

CDKN1C Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The CDKN1C antibody detected the CDKN1C protein (arrow).
CDKN1C Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The CDKN1C antibody detected the CDKN1C protein (arrow).

Immunofluorescence

Fluorescent image of A549 cell stained with CDKN1C Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1C primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). CDKN1C immunoreactivity is localized to Nucleus significantly.
Fluorescent image of A549 cell stained with CDKN1C Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1C primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). CDKN1C immunoreactivity is localized to Nucleus significantly.

Western blot

CDKN1C Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The CDKN1C antibody detected the CDKN1C protein (arrow).
CDKN1C Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The CDKN1C antibody detected the CDKN1C protein (arrow).

Immunofluorescence

Fluorescent image of A549 cell stained with CDKN1C Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1C primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). CDKN1C immunoreactivity is localized to Nucleus significantly.
Fluorescent image of A549 cell stained with CDKN1C Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1C primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). CDKN1C immunoreactivity is localized to Nucleus significantly.

Western blot

CDKN1C Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The CDKN1C antibody detected the CDKN1C protein (arrow).
CDKN1C Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The CDKN1C antibody detected the CDKN1C protein (arrow).

Immunofluorescence

Fluorescent image of A549 cell stained with CDKN1C Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1C primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). CDKN1C immunoreactivity is localized to Nucleus significantly.
Fluorescent image of A549 cell stained with CDKN1C Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1C primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). CDKN1C immunoreactivity is localized to Nucleus significantly.

Western blot

CDKN1C Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The CDKN1C antibody detected the CDKN1C protein (arrow).
CDKN1C Antibody western blot of mouse kidney tissue lysates (35 ug/lane). The CDKN1C antibody detected the CDKN1C protein (arrow).


Requested From: 
Date Requested: 6/21/2018

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