Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Synthetic peptide encompassing the phosphorylated Thr187 residue of human p27.
Specific for p27 when phosphorylated at Thr187. On Western blots, it identifies the target band at ~27kD. It does not react with unphosphorylated p27. Specificity has been confirmed with Thr187-mutant p27 recombinant protein. Reactivity has been confirmed with p27-transfected 293 cells. Based on sequence homology, this antibody may also react with mouse, rat and hamster.
Suitable for use in ELISA and Western Blot. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.