Human CDKN1B / p27 Kip1
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
- Immunofluorescence (5 - 10 µg/ml)
- Western blot (1 - 3 µg/ml)
Synthetic peptide encompassing the phosphorylated Ser10 residue of p27/Kip1.
Recognizes p27 when it is phosphorylated at the Ser10 residue. Lysates of HEK 293T cells transfected with an expression plasmid encoding a p27 mutant where Ser10 was substituted with an alanine residue were used to confirm the antibody's specificity. Reactivity was confirmed with HEK293 cells expressing wild-type p27/KIP that is normally phosphorylated. NIH3T3 and MCF-7 cell lysates can also be used as positive controls. Species cross-reactivity: Human, rat and mouse.
Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Western Blot: 1-3 ug/ml. Immunoprecipitation: 5-10 ug/IP reaction. Immunofluorescence: 5-10 ug/ml.
PBS, pH 7.4, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.