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Anti-CDKN1B / p27 Kip1 Antibody LS-C59248

Catalog Size Price
LS-C59248-100 100 µl Unavailable

Most Popular CDKN1B / p27 Kip1 Antibodies

Anti-CDKN1B / p27 Kip1 Antibody (Ser10) IHC-plus™ LS-B1465
Rabbit Polyclonal (IgG) to Human CDKN1B / p27 Kip1
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CDKN1B / p27 Kip1 Antibody (aa153-165) IHC-plus™ LS-B3943
Goat Polyclonal to Human CDKN1B / p27 Kip1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CDKN1B / p27 Kip1 Antibody (clone 4B4-E6) IHC-plus™ LS-B5389
Mouse Monoclonal [clone 4B4-E6] (IgG1,k) to Human CDKN1B / p27 Kip1
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CDKN1B / p27 Kip1 Antibody (phospho-Thr187) IHC-plus™ LS-B5740
Rabbit Polyclonal to Human CDKN1B / p27 Kip1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CDKN1B / p27 Kip1 Antibody (clone SX53G8) LS-C312129
Mouse Monoclonal [clone SX53G8] (IgG1,k) to Human CDKN1B / p27 Kip1
Human
IHC - Paraffin, IHC - Frozen
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal to Human CDKN1B / p27 Kip1
Human
Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human CDKN1B / p27 Kip1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • Western blot (1:1000 - 1:4000)
  • Immunoprecipitation
  • ELISA (1:10000 - 1:40000)

Specificity and Use

Full length recombinant human p27 protein.
Antiserum will specifically react with a p27 protein from human tissue. No reaction was observed against other related tumor suppressor proteins. No reactivity is observed with p27 from mouse. Reactivity against p27 from other species is unknown
This antibody is suitable for use in ELISA, immunoprecipitation, coimmunoprecipitation and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~27 kD in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. No reactivity is observed with p27 from mouse. This antibody is suitable for immunoprecipitation of in vitro translated protein and cell lysates (C212, 3T3-L1) and will co-precipitate associated proteins. Optimal titers for other applications should be determined by the researcher.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term: -20°C; Long term: -20°C.
For research use only.

About CDKN1B / p27 Kip1

P46527 NM_004064 NP_004055.1

CDKN1B Antibody, KIP1 Antibody, MEN1B Antibody, P27 Kip1 Antibody, p27KIP1 Antibody, MEN4 Antibody, CDKN4 Antibody

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Western blot

Western blot
Anti-p27 Antibody - Western Blot. Western blot of affinity purified anti-p27 antibody shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10000 dilution of Dylight 800 conjugated Gt-a-Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.

Requested From: 
Date Requested: 12/15/2017

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