P27 Kip1 antibody LS-C17116 is an unconjugated mouse monoclonal antibody to p27 Kip1 (CDKN1B) from human, mouse, rat and other species. Validated for Flow, IF, IHC, IP and WB.
Mouse CDKN1B / p27 Kip1
Mouse, Human, Monkey, Rat, Dog
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- IHC - Paraffin
- IHC - Frozen
- Western blot (1:100 - 1:200)
- Flow Cytometry
- (applications tested for the base form of this product only)
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Mouse recombinant p27Kip1 protein.
Highly specific for p27kip1 (27kD). Does not cross-react with other closely related mitotic inhibitors. Co-precipitates cdk4 in complex p27Kip1. Its epitope is mapped between aa83-204 of p27. Species cross-reactivity: Human, monkey, canine, mouse and rat. Does not cross-react with bovine.
Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:100-1:200. Immunohistochemistry: 1:25-1:50 (Frozen, formalin/methacarn, and paraffin embedded sections). Note: Staining of formalin/paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Immunoprecipitation: 10 ul/mg of protein lysate. Positive control: HeLa cells, MAD 109 cells, Ls174T cells, breast carcinoma or colon carcinoma.
PBS, pH 7.4, 7.5 mM Sodium Azide, 40% Glycerol, 0.1% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.