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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDKN1B / p27 Kip1 Antibody (aa83-204)

P27 Kip1 antibody LS-C17116 is an unconjugated mouse monoclonal antibody to p27 Kip1 (CDKN1B) from human, mouse, rat and other species. Validated for Flow, IF, IHC, IP and WB.
Catalog
Size
Price
LS-C17116-100
100 µg
$635
Description
P27 Kip1 antibody LS-C17116 is an unconjugated mouse monoclonal antibody to p27 Kip1 (CDKN1B) from human, mouse, rat and other species. Validated for Flow, IF, IHC, IP and WB.
Target
Mouse CDKN1B / p27 Kip1
Host
Mouse
Reactivity
Mouse, Human, Monkey, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Immunofluorescence
  • Western blot (1:100 - 1:200)
  • Immunoprecipitation
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Mouse recombinant p27Kip1 protein.
Epitope
aa83-204
Specificity
Highly specific for p27kip1 (27kD). Does not cross-react with other closely related mitotic inhibitors. Co-precipitates cdk4 in complex p27Kip1. Its epitope is mapped between aa83-204 of p27. Species cross-reactivity: Human, monkey, canine, mouse and rat. Does not cross-react with bovine.
Usage
Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:100-1:200. Immunohistochemistry: 1:25-1:50 (Frozen, formalin/methacarn, and paraffin embedded sections). Note: Staining of formalin/paraffin sections requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling at room temperature for 20 minutes. Immunoprecipitation: 10 ul/mg of protein lysate. Positive control: HeLa cells, MAD 109 cells, Ls174T cells, breast carcinoma or colon carcinoma.
Presentation
PBS, pH 7.4, 7.5 mM Sodium Azide, 40% Glycerol, 0.1% BSA
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CDKN1B / p27 Kip1
P46527 NM_004064 NP_004055.1

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Requested From: United States
Date Requested: 1/23/2019
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