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Antibodies > CDKN1B / p27 Kip1 > LS-C343947
 
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Catalog Number Size Price
LS-C343947-10 10 µg $318 
LS-C343947-100 100 µg (0.5 mg/ml) $470 
CDKN1B / p27 Kip1 Antibody - IHC analysis of p27 KIP 1 using anti-p27 KIP 1 antibody. p27 KIP 1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-p27 KIP 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDKN1B / p27 Kip1 Antibody - IHC analysis of p27 KIP 1 using anti-p27 KIP 1 antibody. p27 KIP 1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-p27 KIP 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDKN1B / p27 Kip1 Antibody - IHC analysis of p27 KIP 1 using anti-p27 KIP 1 antibody. p27 KIP 1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-p27 KIP 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDKN1B / p27 Kip1 Antibody - IHC analysis of p27 KIP 1 using anti-p27 KIP 1 antibody. p27 KIP 1 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-p27 KIP 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDKN1B / p27 Kip1 Antibody - IF analysis of p27 KIP 1 using anti-p27 KIP 1 antibody p27 KIP 1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-p27 KIP 1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CDKN1B / p27 Kip1 Antibody - P27 KIP 1 antibody Western blot. All lanes: Anti-p27 KIP 1 at 0.5 ug/ml. Lane 1: Rat Thymus Tissue Lysate at 40 ug. Lane 2: Rat Brain Tissue Lysate at 40 ug. Predicted band size: 27 kD. Observed band size: 27 kD.
CDKN1B / p27 Kip1 Antibody - Flow Cytometry analysis of SiHa cells using anti-p27 KIP 1 antibody. Overlay histogram showing SiHa cells stained with anti-p27 KIP 1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p27 KIP 1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CDKN1B / p27 Kip1 Antibody - IHC analysis of p27 KIP 1 using anti-p27 KIP 1 antibody. p27 KIP 1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-p27 KIP 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDKN1B / p27 Kip1 Antibody - IHC analysis of p27 KIP 1 using anti-p27 KIP 1 antibody. p27 KIP 1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-p27 KIP 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDKN1B / p27 Kip1 Antibody - IHC analysis of p27 KIP 1 using anti-p27 KIP 1 antibody. p27 KIP 1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-p27 KIP 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDKN1B / p27 Kip1 Antibody - IHC analysis of p27 KIP 1 using anti-p27 KIP 1 antibody. p27 KIP 1 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-p27 KIP 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDKN1B / p27 Kip1 Antibody - IF analysis of p27 KIP 1 using anti-p27 KIP 1 antibody p27 KIP 1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-p27 KIP 1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CDKN1B / p27 Kip1 Antibody - P27 KIP 1 antibody Western blot. All lanes: Anti-p27 KIP 1 at 0.5 ug/ml. Lane 1: Rat Thymus Tissue Lysate at 40 ug. Lane 2: Rat Brain Tissue Lysate at 40 ug. Predicted band size: 27 kD. Observed band size: 27 kD.
CDKN1B / p27 Kip1 Antibody - Flow Cytometry analysis of SiHa cells using anti-p27 KIP 1 antibody. Overlay histogram showing SiHa cells stained with anti-p27 KIP 1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p27 KIP 1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human CDKN1B / p27 Kip1 Antibody (aa10‑198, WB) LS‑C343947

Polyclonal Rabbit anti‑Human CDKN1B / p27 Kip1 Antibody (aa10‑198, WB) LS‑C343947

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Note: This antibody replaces LS-C358005
Antibody:
CDKN1B / p27 Kip1 Rabbit anti-Human Polyclonal (aa10-198) Antibody
Application:
WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C343947-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CDKN1B / p27 Kip1 Rabbit anti-Human Polyclonal (aa10-198) Antibody
Application:
WB
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
P27 Kip1 antibody LS-C343947 is an unconjugated rabbit polyclonal antibody to p27 Kip1 (CDKN1B) (aa10-198) from human. It is reactive with human and rat. Validated for WB.
Target
Human CDKN1B / p27 Kip1
Synonyms
CDKN1B | KIP1 | MEN1B | P27 Kip1 | p27KIP1 | MEN4 | CDKN4
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human P27 KIP 1 recombinant protein (Position: S10-T198). Human P27 KIP 1 shares 87% amino acid (aa) sequence identity with mouse P27 KIP 1.
Epitope
aa10-198
Specificity
Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDKN1B / p27 Kip1
P46527 NM_004064 NP_004055.1

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Featured Products

CDKN1B / p27 Kip1 Antibody - Anti-CDKN1B / p27 Kip1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ CDKN1B / p27 Kip1 Antibody (aa153‑165) LS‑B3943
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
CDKN1B / p27 Kip1 Antibody - Anti-CDKN1B / p27 Kip1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ CDKN1B / p27 Kip1 Antibody (clone 4B4‑E6) LS‑B5389
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
CDKN1B / p27 Kip1 Antibody - The anti-Phospho-p27Kip1-T187antibody is used in Western blot to detect Phospho-p27Kip1-T187 in HL60 tissue lysate
CDKN1B / p27 Kip1 Antibody (phospho‑Thr187) LS‑C96931
Species: Human
Applications: Western blot
CDKN1B / p27 Kip1 Antibody - IHC of p27 on FFPE Breast Adenocarcinoma tissue.
CDKN1B / p27 Kip1 Antibody (clone SX53G8) LS‑C312129
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen
CDKN1B / p27 Kip1 Antibody - Dot blot of Mouse p27Kip1 Antibody (Phospho T197) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
CDKN1B / p27 Kip1 Antibody (phospho‑Thr197) LS‑C157925
Species: Mouse
Applications: Dot Blot
CDKN1B / p27 Kip1 Antibody - Western blot analysis of lysates from HeLa cells treated with EGF or IFN-α, using p27 Kip1 (Phospho-Thr187) Antibody. The lane on the left is blocked with the phospho peptide.
CDKN1B / p27 Kip1 Antibody (phospho‑Thr187) LS‑C117439
Species: Human, Mouse, Rat
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all CDKN1B / p27 Kip1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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