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Anti-CDKN1A / WAF1 / p21 Antibody (clone WA-1) LS-C59514


Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular CDKN1A / WAF1 / p21 Antibodies

Anti-CDKN1A / WAF1 / p21 Antibody (aa117-146) LS-C168621
Rabbit Polyclonal to Human CDKN1A / WAF1 / p21
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (5)
Mouse Monoclonal [clone WA-1] to Human CDKN1A / WAF1 / p21
IHC - Paraffin, IHC - Frozen, Western blot, ELISA


Human CDKN1A / WAF1 / p21
Human (tested or 100% immunogen sequence identity)
Monoclonal [WA-1]
Protein A purified


  • IHC - Paraffin
  • IHC - Frozen
  • Western blot

Specificity and Use

CDKN1A / WAF1 / p21 antibody was raised against recombinant human p21 fusion protein.
human and mammalian p21. The antibody is highly specific and shows no cross-reactivity with other closely related mitotic inhibitors. The intracellular protein p21 is a 21 kD protein and also known as wild-type p53-activated fragment 1 (WAF1). It is an inhibitor of cyclin-dependent kinases (Cdks) and proliferating-cell nuclear antigen (PCNA)
This antibody is suitable for immunohistochemistry, ELISA and immunoblotting. The antibody detects a 21 kD band corresponding to the p21 protein. MCF7 cells or UV treated fibroblasts are suggested as a positive control for immunoblotting and immunohistochemistry. Methanol/acetone fixed tissue is reactive for immunohistochemistry. The antibody also reacts with breast carcinoma on frozen sections (methanol/acetone fixation) and on paraffin sections (formalin fixation and microwave treatment prior to staining). In ELISA this monoclonal antibody is useful either as a capture antibody (attached to the solid phase) or for detection of p21 protein. This antibody immunoprecipitates monomeric p21 WAF1 and not p21 complexes with other proteins. High level expression of p21 WAF1 requires induction of wild-type p53 expression (e. g. via exposure to DNA damaging agents).


0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

Publications (5)

The p21 cyclin-dependent kinase inhibitor suppresses tumorigenicity in vivo. Yang ZY, Perkins ND, Ohno T, Nabel EG, Nabel GJ. Nature medicine. 1995 1:1052-6. [PubMed:7489362]
p21 is necessary for the p53-mediated G1 arrest in human cancer cells. Waldman T, Kinzler KW, Vogelstein B. Cancer research. 1995 55:5187-90. [PubMed:7585571]
A cis-acting element in Rous sarcoma virus long terminal repeat required for promoter repression by HeLa nuclear protein p21. Yeh CH, Shatkin AJ. The Journal of biological chemistry. 1995 270:15815-20. [PubMed:7797584]
p53 expression overcomes p21WAF1/CIP1-mediated G1 arrest and induces apoptosis in human cancer cells. Kagawa S, Fujiwara T, Hizuta A, Yasuda T, Zhang WW, Roth JA, Tanaka N. Oncogene. 1997 15:1903-9. [PubMed:9365236]
Inhibition of tumor cell growth by p21WAF1 adenoviral gene transfer in lung cancer. Joshi US, Chen YQ, Kalemkerian GP, Adil MR, Kraut M, Sarkar FH. Cancer gene therapy. 5:183-91. [PubMed:9622102]

About CDKN1A / WAF1 / p21

P38936 NM_078467 NP_000380.1

CDKN1A Antibody, CDKN1 Antibody, CIP1 Antibody, CDK-interacting protein 1 Antibody, DNA synthesis inhibitor Antibody, p21CIP1 Antibody, MDA6 Antibody, PIC1 Antibody, SDI1 Antibody, WAF1 Antibody, CAP20 Antibody, CDK-interaction protein 1 Antibody, MDA-6 Antibody, p21 Antibody

CDKN1A / WAF1 / p21 is a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli.

Western blot

Western blot
Anti-Human p21WAF1 Monoclonal Antibody - Western Blot. Mab anti-Human p21WAF1 antibody (clone WA-1) is shown to detect human p21 by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1000 dilution of Mab anti-Human p21WAF1at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] ( diluted 1:5000. A single band corresponding to human p21WAF1 is detected at ~21 kD when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p21WAF1. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 10/25/2016

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