Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human CDKN1A / WAF1 / p21
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
Mouse Monoclonal [clone B459] (IgG1) to Human CDKN1A / WAF1 / p21
IHC, Western blot, ELISA
Human CDKN1A / WAF1 / p21
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [B459]
IHC (1:50 - 1:100)
Western blot (1:100 - 1:300)
ELISA (1:500 - 1:3000)
Specificity and Use
Human p21 protein.
This antibody reacts with human and other mammalian p21 protein. p21 is an intracellular protein of 21kD size. It is also known as the wild type p53 activated fragment 1 or WAF1. p21 inhibits cyclin-dependent kinases (CdkÃ¢‚¬„¢s) and also proliferating cell nuclear antigens or PCNAÃ¢‚¬„¢s).
20 mM Tris-borate, 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM, Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, 0.05% sodium azide, pH 7.5
CDKN1A / WAF1 / p21 is a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli.