Human CDK9

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Mouse, Rat (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T29 in the human CDK9 protein.

pThr29

This antibody is specific for human CDK9 protein phosphorylated at T29. A BLAST analysis was used to suggest cross-reactivity with CDK9 from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with CDK9 from other sources has not been determined.

• Western blot (1:200 - 1:2000)
• Immunoprecipitation (1:100)
• ELISA (1:5000 - 1:24000)

This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 42 kD in size corresponding to phosphorylated CDK9 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human CDK9 pT29 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: -20°C; Long term: -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P50750 NCBI: NM_001261 NP_001252.1