Target
Human CDK5
Synonyms
CDK5 | CDKN5 | Cell division protein kinase 5 | Crk6 | Cyclin-dependent kinase 5 | Protein kinase CDK5 splicing | PSSALRE | TPKII catalytic subunit
Reactivity
Human, Mouse, Rat, Drosophila
(tested or 100% immunogen sequence identity)
Clonality
IgG2a
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
Recombinant human cdk5.
Specificity
Recognizes human cdk5, Mr 31kD. Species cross-reactivity: mouse, rat and Drosophila.
Applications
- ICC (5 µg/ml)
- Western blot
- Immunoprecipitation
- ELISA
Usage
Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and ELISA. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots of antibody detected cdk5 in RIPA lysates from mouse Wehi cells and rat PC12 cells. Immunoprecipitation: 4 ug/500 ug of A431 cell lysate. Immunocytochemistry: 5 ug/ml stains cdk5 in human A431 cells fixed with 4% paraformaldehyde.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee