CDK5 antibody LS-C22052 is an unconjugated mouse monoclonal antibody to CDK5 from human, mouse, rat and other species. Validated for ELISA, ICC, IP and WB.
Human, Mouse, Rat, Drosophila
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- ICC (5 µg/ml)
- Western blot
- (applications tested for the base form of this product only)
CDK5 antibody was raised against recombinant human cdk5.
Recognizes human cdk5, Mr 31kD. Species cross-reactivity: mouse, rat and Drosophila.
Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and ELISA. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Previous lots of antibody detected cdk5 in RIPA lysates from mouse Wehi cells and rat PC12 cells. Immunoprecipitation: 4 ug/500 ug of A431 cell lysate. Immunocytochemistry: 5 ug/ml stains cdk5 in human A431 cells fixed with 4% paraformaldehyde.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.