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| Catalog Number | Size | Price |
|---|---|---|
| LS-B353-50 | 50 µl (85 mg/ml) | $515 |
IHC‑plus™ Polyclonal Rabbit anti‑Human CDK5 Antibody (C‑Terminus, IHC, WB) LS‑B353
IHC‑plus™ Polyclonal Rabbit anti‑Human CDK5 Antibody (C‑Terminus, IHC, WB) LS‑B353
Note: This antibody replaces LS-C18775
Antibody:
CDK5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CDK5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
CDK5 antibody LS-B353 is an unconjugated rabbit polyclonal antibody to CDK5 (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human CDK5
Synonyms
CDK5 | CDKN5 | Cell division protein kinase 5 | Crk6 | Cyclin-dependent kinase 5 | Protein kinase CDK5 splicing | PSSALRE | TPKII catalytic subunit
Host
Rabbit
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
CDK5 (p31) peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Epitope
C-Terminus
Specificity
Antiserum will specifically react with a 35 kDa cdk5 (p31) protein from human, rat and mouse tissue. No reaction was observed against other related cyclin dependent kinases. Cross reactivity with cdk5 (p31) from other species may also occur.
Applications
- IHC
- IHC - Paraffin (1:500)
- Western blot (1:500 - 1:5000)
- Immunoprecipitation (1:100)
- ELISA (1:5000 - 1:20000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B353 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B353 was determined to be 1:500.
Presentation
Antiserum, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CDK5
Validation
Anti-CDK5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
Anti-CDK5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
See More About...
LSBio Ratings
IHC-plus™ CDK5 Antibody (C-Terminus) for IHC, WB/Western, IP, ELISA LS-B353 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
CDK5 positively regulates Notch1 signaling in pancreatic cancer cells by phosphorylation. Qiaoyun Chu, Liyong Wang, Jie Zhang, Wei Wang, Youxin Wang. Cancer medicine. 2021 Jun;;10:3689-3699.
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Drosophila
Applications:
ICC, Western blot, Immunoprecipitation, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
