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Anti-CDK4 Antibody (clone 4F11) LS-C196880

Catalog Size Price
LS-C196880-100 100 µg Unavailable

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100% Guaranteed
Mouse Monoclonal [clone 4F11] (IgG1,k) to Human CDK4
Human
Immunofluorescence, Western blot, ELISA, RNA interference
Unconjugated

Details

Human CDK4
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [4F11]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
  • RNA interference

Specificity and Use

CDK4 antibody was raised against cDK4 (AAH03644, aa211-303 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human CDK4
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About CDK4

P11802 NM_000075 NP_000066.1

CDK4 Antibody, Cell division protein kinase 4 Antibody, Crk3 Antibody, Cyclin-dependent kinase 4 Antibody, CMM3 Antibody, p34/cdk4 Antibody, PSK-J3 Antibody

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
Western Blot analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11.Lane 1: CDK4 transfected lysate(33.7 KDa).Lane 2: Non-transfected lysate.

Western blot

Western blot
CDK4 monoclonal antibody (M03), clone 4F11. Western Blot analysis of CDK4 expression in 293.

Western blot

Western blot
CDK4 monoclonal antibody (M03), clone 4F11. Western Blot analysis of CDK4 expression in HeLa NE.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged CDK4 is approximately 1 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot analysis of CDK4 over-expressed 293 cell line, cotransfected with CDK4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDK4 monoclonal antibody (M03) clone 4F11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Requested From: 
Date Requested: 6/25/2017

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