Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase.
Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of CDK4 expression in transfected 293T cell line by CDK4 monoclonal antibody (M03), clone 4F11.Lane 1: CDK4 transfected lysate(33.7 KDa).Lane 2: Non-transfected lysate.
CDK4 monoclonal antibody (M03), clone 4F11. Western Blot analysis of CDK4 expression in 293.
CDK4 monoclonal antibody (M03), clone 4F11. Western Blot analysis of CDK4 expression in HeLa NE.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged CDK4 is approximately 1 ng/ml as a capture antibody.
Western blot analysis of CDK4 over-expressed 293 cell line, cotransfected with CDK4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDK4 monoclonal antibody (M03) clone 4F11 . GAPDH ( 36.1 kDa ) used as specificity and loading control.