Target
Human CDK2
Synonyms
CDK2 | Cell division protein kinase 2 | CDC2-related protein kinase | p33 protein kinase | p33(CDK2) | CDKN2 | Cyclin-dependent kinase 2
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr160 of human cdk2 (KLH coupled).
Specificity
Detects cdk2 only when phosphorylated at threonine 160. Weakly cross-reacts with cdc2 phosphorylated at Thr161. Does not cross-react with other phosphorylated cyclin-dependent kinases. Species cross-reactivity: Human, mouse and rat.
Applications
- Western blot (1:1000)
- Immunoprecipitation (1:150)
- ELISA
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:150.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee