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Anti-CDK2 Antibody LS-C5973

Ordering

Wt. Vol. Conc. Price
100 µg - 0.2 mg/ml $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Mouse Monoclonal (IgG2b) to Human CDK2
Human, Mouse, Rat
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated

Details

Human CDK2
Mouse
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG2b Monoclonal
Protein A purified

Applications

IHC - Paraffin (2 - 4 µg/ml), IHC - Frozen (2 - 4 µg/ml), Western blot (1 µg/ml), Immunoprecipitation

Specificity and Use

CDK2 antibody was raised against purified recombinant human cdk2 protein.
Recognizes human cyclin-dependent kinase-2 (cdk2) at 33kD. Species cross-reactivity: Mouse, and rat. Does not cross-react with other members of the cdk family.
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation: 10 ul/mg; co-precipitates associated proteins. Western Blot: 1 ug/ml Incubate for 2 hours at RT. Immunohistochemistry: 2-4 ug/ml cryostat sections and paraffin embedded sections. For paraffin embedded sections, boil the sections in 1 mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min. Positive control: HeLa, 293, VA13, IMR90, Jurkat and WI-38 cells; thymus, tonsil or reactive lymph node.

Packaging

PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About CDK2

P24941 NM_001798 NP_001789.2

CDK2 Antibody, Cell division protein kinase 2 Antibody, CDC2-related protein kinase Antibody, p33 protein kinase Antibody, p33(CDK2) Antibody, CDKN2 Antibody, Cyclin-dependent kinase 2 Antibody

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA.

Requested From: United States
Date Requested: 9/25/2016

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