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Anti-CDK2 Antibody (clone AN4.3) IHC-plus™ LS-B2483

Note: This antibody replaces LS-C5978

Catalog	Size	Price
LS-B2483-50	50 µg (0.85 mg/ml)	Unavailable

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Reactivity:	Human, Mouse, Rat
Application:	IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Format:	Unconjugated

Anti-CDK2 Antibody LS-C5973

Antibody:	Mouse Monoclonal (IgG2b) to Human CDK2
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Format:	Unconjugated

Anti-CDK2 Antibody (C-Terminus) IHC-plus™ LS-B352

Antibody:	Rabbit Polyclonal to Human CDK2
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Anti-CDK2 Antibody IHC-plus™ LS-B2482

Antibody:	Mouse Monoclonal (IgG2,k) to Human CDK2
Reactivity:	Human, Mouse, Rat
Application:	IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Format:	Unconjugated

Anti-CDK2 Antibody (clone AN4.3) IHC-plus™ LS-B2483

Antibody:	Mouse Monoclonal [clone AN4.3] (IgG) to Human CDK2
Reactivity:	Human, Mouse, Xenopus
Application:	IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Format:	Unconjugated

Antibody:	Mouse Monoclonal [clone AN4.3] (IgG) to Human CDK2
Reactivity:	Human, Mouse, Xenopus
Application:	IHC - Paraffin, Western blot, Immunoprecipitation, ELISA (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human CDK2
Host:	Mouse
Reactivity:	Human, Mouse, Xenopus (tested or 100% immunogen sequence identity)
Clonality:	IgG Monoclonal [AN4.3]
Conjugations:	Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Purification:	Protein G purified
Modifications:	Unmodified

Applications

Approved:

IHC - Paraffin (5 µg/ml)
Western blot
Immunoprecipitation
ELISA
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:	CDK2 antibody was raised against recombinant human cdk2.
Specificity:	Recognizes human cdk2, MW 33kD. Does not cross-react with other cdks. Species cross-reactivity: mouse and Xenopus.
Usage:	Immunohistochemistry: LS-B2483 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2483 was determined to be 5 ug/ml.

Packaging

Presentation:	0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%
Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Restrictions:	For research use only.

About CDK2

Uniprot: P24941 NCBI: NM_001798 NP_001789.2

Synonyms: CDK2 Antibody, Cell division protein kinase 2 Antibody, CDC2-related protein kinase Antibody, p33 protein kinase Antibody, p33(CDK2) Antibody, CDKN2 Antibody, Cyclin-dependent kinase 2 Antibody

Description: Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA.

(More About CDK2)

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Immunohistochemistry

Anti-CDK2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2483 concentration 5 ug/ml.

Requested From:
Date Requested: 7/22/2017

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