Target
Human CDK1 / CDC2
Synonyms
CDK1 | CDC28A | CDC2 | CDKN1 | Cyclin-dependent kinase 1 | p34 protein kinase | p34CDC2 | CDC2a | Cell cycle controller CDC2 | Cell division protein kinase 1 | PSTAIR
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr161 of human cdc2 (KLH coupled).
Specificity
Detects endogenous levels of cdc2 only when phosphorylated at Thr161. Cross-reacts with endogenous cdk2 phosphorylated at Thr160. Species cross-reactivity: Mouse and rat.
Applications
- ICC
- Immunofluorescence
- Western blot (1:1000)
- Immunoprecipitation (1:50)
- ELISA
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunofluorescence. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunocytochemistry (for IF): 1:200.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee