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Anti-CDK1 / CDC2 Antibody (clone POH-1) LS-C18772


Wt. Vol. Conc. Price
100 µg - 1 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular CDK1 / CDC2 Antibodies

Anti-CDK1 / CDC2 Antibody (Thr161) IHC-plus™ LS-B1179
Rabbit Polyclonal (IgG) to Human CDK1 / CDC2
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Image
Anti-CDK1 / CDC2 Antibody (Tyr15) IHC-plus™ LS-B1180
Rabbit Polyclonal (IgG) to Human CDK1 / CDC2
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Image
Anti-CDK1 / CDC2 Antibody (clone 26E11) IHC-plus™ LS-B7092
Mouse Monoclonal [clone 26E11] (IgG2a,k) to Human CDK1 / CDC2
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (3)
Mouse Monoclonal [clone POH-1] to Human CDK1 / CDC2
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation


Human CDK1 / CDC2
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Monoclonal [POH-1]
Protein A purified


  • IHC - Paraffin
  • Western blot
  • Immunoprecipitation

Specificity and Use

CDK1 / CDC2 antibody was raised against recombinant human CDK1.
Recombinant human p34 cdc2 fusion protein.
This antibody is suitable for immunohistochemistry immunoprecipitation (as active kinase), and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products. HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting. LEP fibroblast cell lysate is suggested as a negative control. Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1 % saponin treatment or other permeabilization method.


0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (3)

Distinct forms of human CDC2 identified by novel monoclonal antibodies. Luks J, Draetta G, Bartek J. European journal of biochemistry / FEBS. 1992 207:169-76. [PubMed:1628647]
Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2. Ye XS, Fincher RR, Tang A, McNeal KK, Gygax SE, Wexler AN, Ryan KB, James SW, Osmani SA. The Journal of biological chemistry. 1997 272:33384-93. [PubMed:9407133]
A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1. Palmer A, Gavin AC, Nebreda AR. The EMBO journal. 1998 17:5037-47. [PubMed:9724639] [PMC:PMC1170831]

About CDK1 / CDC2

P06493 NM_001786 NP_001777.1

CDK1 Antibody, CDC28A Antibody, CDC2 Antibody, CDKN1 Antibody, Cyclin-dependent kinase 1 Antibody, p34 protein kinase Antibody, p34CDC2 Antibody, CDC2a Antibody, Cell cycle controller CDC2 Antibody, Cell division protein kinase 1 Antibody, PSTAIR Antibody

Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis.

Western blot

Western blot
Mab anti-Human p34Cdc2 antibody was used to detect human p34Cdc2by Western blot of untreated (control) and drug treated (10 M genistein) lysates of MCF-7 cells. Very strong detection occurs using a 1:1000 dilution. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.

Western blot

Western blot
Mab anti-Human p34°Cdc2 antibody (clone POH-1) is shown to detect human p34°Cdc2by western blot. Detection occurs after 10 ug of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1000 dilution of Mab anti-Human p34°Cdc2at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5000. A doublet band corresponding to human p34°Cdc2is detected at ~34 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p34°Cdc2. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot

Western blot
Mab anti-Human p34cdc2 antibody was used at a dilution of 1:1000 to detect human p34cdc2by Western blot of untreated (control) and drug treated (10 mM genistein) lysates of MCF-7 cells.

Requested From: United States
Date Requested: 10/24/2016

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