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Catalog Number Size Price
LS-C407746-10 10 µg $318 
LS-C407746-100 100 µg $470 
CDK1 / CDC2 Antibody - CDK1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
CDK1 / CDC2 Antibody - CDK1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.
CDK1 / CDC2 Antibody - CDK1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.
CDK1 / CDC2 Antibody - IF analysis of CDK1 using anti-CDK1 antibody CDK1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CDK1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CDK1 / CDC2 Antibody - CDK1 antibody Western blot. All lanes: Anti CDK1 at 0.5 ug/ml. Lane 1: Rat Thymus Tissue Lysate at 50 ug. Lane 2: Rat Spleen Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Lane 5: JURKAT Whole Cell Lysate at 40 ug. Lane 6: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 34 kD. Observed band size: 34 kD.
CDK1 / CDC2 Antibody - Flow Cytometry analysis of U937 cells using anti-CDK1 antibody. Overlay histogram showing U937 cells stained with anti-CDK1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDK1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CDK1 / CDC2 Antibody - CDK1 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.
CDK1 / CDC2 Antibody - CDK1 antibody IHC-paraffin. IHC(P): Rat Testis Tissue.
CDK1 / CDC2 Antibody - CDK1 antibody IHC-paraffin. IHC(P): Mouse Testis Tissue.
CDK1 / CDC2 Antibody - IF analysis of CDK1 using anti-CDK1 antibody CDK1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CDK1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CDK1 / CDC2 Antibody - CDK1 antibody Western blot. All lanes: Anti CDK1 at 0.5 ug/ml. Lane 1: Rat Thymus Tissue Lysate at 50 ug. Lane 2: Rat Spleen Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Lane 5: JURKAT Whole Cell Lysate at 40 ug. Lane 6: NIH3T3 Whole Cell Lysate at 40 ug. Predicted band size: 34 kD. Observed band size: 34 kD.
CDK1 / CDC2 Antibody - Flow Cytometry analysis of U937 cells using anti-CDK1 antibody. Overlay histogram showing U937 cells stained with anti-CDK1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDK1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human CDK1 / CDC2 Antibody (aa66‑297, IHC, WB) LS‑C407746

Polyclonal Rabbit anti‑Human CDK1 / CDC2 Antibody (aa66‑297, IHC, WB) LS‑C407746

Antibody:
CDK1 / CDC2 Rabbit anti-Human Polyclonal (aa66-297) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407746-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CDK1 / CDC2 Rabbit anti-Human Polyclonal (aa66-297) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CDC2 antibody LS-C407746 is an unconjugated rabbit polyclonal antibody to CDC2 (CDK1) (aa66-297) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human CDK1 / CDC2
Synonyms
CDK1 | CDC28A | CDC2 | CDKN1 | Cyclin-dependent kinase 1 | p34 protein kinase | p34CDC2 | CDC2a | Cell cycle controller CDC2 | Cell division protein kinase 1 | PSTAIR
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human CDK1 recombinant protein (Position: L66-M297). Human CDK1 shares 97.8% and 98.3% amino acid (aa) sequence identity with mouse and rat CDK1, respectively.
Epitope
aa66-297
Specificity
Isoform 2 is found in breast cancer tissues.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDK1 / CDC2
P06493 NM_001786 NP_001777.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/25/2024