Human CDH2 / N Cadherin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

13A9

Unconjugated

Protein G purified

Unmodified

Recombinant MBP fusion protein containing the entire cytoplasmic domain of human N-cadherin.

Cytoplasmic Domain

Recognizes neural cadherin, otherwise known as CD325, a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry. N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells. Studies using clone 13A9 have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin see for details. Clone 13A9 has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin, see for details. Immunohistological studies have shown that clone 13A9 can be used as a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarcinomas, when used in conjunction with E-cadherin see for details.

• IHC - Paraffin
• IHC - Frozen
• Immunofluorescence
• Western blot (1:100 - 1:1000)
• Immunoprecipitation

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P19022 NCBI: NM_001792 NP_001783.2