Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence.
N-Cadherin in Human Brain. N-Cadherin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes.
N-Cadherin in A549 Human Cell Line. N-Cadherin was detected in immersion fixed A549 human lung carcinoma cell line using Human/Mouse/Rat N-Cadherin Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces.
Detection of Human, Mouse, and Rat N-Cadherin by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and rat brain tissue. PVDF Membrane was probed with 0.5 ug/ml of Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for N-Cadherin at approximately 130 kD (as indicated). This experiment was conducted under reducing conditions.
Detection of N-Cadherin in HeLa Human Cell Line by Flow Cytometry. He La human cervical epithelial carcinoma cell line was stained with Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody or Sheep IgG control antibody, followed by NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody.
Requested From: United States
Date Requested: 10/21/2016