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Anti-CDH2 / N Cadherin Antibody (aa160-724) LS-C150135

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CDH2 / N Cadherin Antibodies

Anti-CDH2 / N Cadherin Antibody (clone 8C11, PE) LS-C106514
Mouse Monoclonal [clone 8C11] (IgG1,k) to Human CDH2 / N Cadherin
Human
Flow Cytometry
PE Conjugated
Flow Cytometry Image
Anti-CDH2 / N Cadherin Antibody (Cytoplasmic Domain, clone 13A9) LS-C143420
Mouse Monoclonal [clone 13A9] (IgG1) to Human CDH2 / N Cadherin
Human, Mouse
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunocytochemistry Image
Anti-CDH2 / N Cadherin Antibody (clone 2G7) LS-C173526
Mouse Monoclonal [clone 2G7] (IgG2a) to Human CDH2 / N Cadherin
Human, Monkey, Mouse, Dog
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human CDH2 / N Cadherin
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot, Flow Cytometry
Unconjugated

Details

Human CDH2 / N Cadherin
Sheep
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • ICC (5 - 15 µg/ml)
  • Western blot (0.5 µg/ml)
  • Flow Cytometry

Specificity and Use

CDH2 / N Cadherin antibody was raised against mouse myeloma cell line NS0-derived recombinant human N-Cadherin Asp160-Ala724 Accession # P19022.
aa160-724
Detects human, mouse, and rat N-Cadherin in Western blots.
Western Blot, 0.5 µg/ml. Flow Cytometry, 2.5 µg/106 cells. Immunocytochemistry, 5-15 µg/ml. Immunohistochemistry, 5-15 µg/ml.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About CDH2 / N Cadherin

P19022 NM_001792 NP_001783.2

CDH2 Antibody, CDw325 Antibody, CDHN Antibody, Cadherin-2 Antibody, CD325 Antibody, CD325 antigen Antibody, N Cadherin Antibody, N-cadherin Antibody, NCAD Antibody, Neural cadherin Antibody, N-cadherin 1 Antibody, Neural-cadherin Antibody

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence.

Immunohistochemistry

Immunohistochemistry
N-Cadherin in Human Brain. N-Cadherin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes.

Immunocytochemistry

Immunocytochemistry
N-Cadherin in A549 Human Cell Line. N-Cadherin was detected in immersion fixed A549 human lung carcinoma cell line using Human/Mouse/Rat N-Cadherin Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces.

Western blot

Western blot
Detection of Human, Mouse, and Rat N-Cadherin by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and rat brain tissue. PVDF Membrane was probed with 0.5 ug/ml of Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for N-Cadherin at approximately 130 kD (as indicated). This experiment was conducted under reducing conditions.

Flow Cytometry

Flow Cytometry
Detection of N-Cadherin in HeLa Human Cell Line by Flow Cytometry. He La human cervical epithelial carcinoma cell line was stained with Human/Mouse/Rat N-Cadherin Antigen Affinity-purified Polyclonal Antibody or Sheep IgG control antibody, followed by NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody.

Requested From: United States
Date Requested: 12/4/2016

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