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Anti-CDH15 / M Cadherin Antibody (aa22-606) LS-C149994

Ordering

Wt. Vol. Conc. Price
100 µg - 0.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CDH15 / M Cadherin Antibodies

Anti-CDH15 / M Cadherin Antibody (Extracellular Domain) LS-C127165
Mouse Monoclonal (IgG1) to Human CDH15 / M Cadherin
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
Anti-CDH15 / M Cadherin Antibody (aa22-606) LS-C149994
Sheep Polyclonal (IgG) to Human CDH15 / M Cadherin
Human
ICC, Western blot, Flow Cytometry
Unconjugated
Immunocytochemistry Image
Anti-CDH15 / M Cadherin Antibody (C-Terminus, Biotin) LS-C275750
Rabbit Polyclonal (IgG) to Human CDH15 / M Cadherin
Human
IHC, Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human CDH15 / M Cadherin
Human
ICC, Western blot, Flow Cytometry
Unconjugated

Details

Human CDH15 / M Cadherin
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Flow Cytometry

Specificity and Use

CDH15 / M Cadherin antibody was raised against mouse myeloma cell line NS0-derived recombinant human M-Cadherin/Cadherin15 Val22-Ala606 Accession # P55291.
aa22-606
Detects human M-Cadherin/Cadherin-15 in direct ELISAs and Western blots.
Western Blot, 1 µg/ml. Flow Cytometry, 2.5 µg/106 cells. Immunocytochemistry, 5-15 µg/ml.

Packaging

Lyophilized from 0.2 µm filtered PBS, pH 7.4, 5% trehalose
Reconstitute with sterile PBS.
Long term: -70°C; Short term: -20°C
For research use only.

About CDH15 / M Cadherin

P55291 NM_004933 NP_004924.1

CDH15 Antibody, Cadherin-14 Antibody, Cadherin-3 Antibody, CDH14 Antibody, M-cadherin Antibody, Muscle cadherin Antibody, MRD3 Antibody, Muscle-cadherin Antibody, M Cadherin Antibody, Cadherin-15 Antibody, CDHM Antibody, MCAD Antibody, Myotubule-cadherin Antibody

CDH15 / M Cadherin is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells.

Immunocytochemistry

Immunocytochemistry
M-Cadherin/Cadherin-15 in C2C12 Mouse Cell Line. M-Cadherin/Cadherin-15 was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Human M-Cadherin/Cadherin-15

Western blot

Western blot
Detection of Human M Cadherin/Cadherin 15 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 ug/ml of Sheep Anti-Human M-Cadherin/Cadherin15Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for M Cadherin/Cadherin15 at approximately130 kD (as indicated). This experiment was conducted under reducing conditions.

Flow Cytometry

Flow Cytometry
Detection of M-Cadherin/ Cadherin-15 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Sheep Anti-Human M-Cadherin/ Cadherin-15 Antigen Affinity-purified Polyclonal Antibody or control antibody, followed by NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody.
 

Requested From: United States
Date Requested: 12/8/2016

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