Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1) to Human CDH1 / E Cadherin
IHC - Paraffin
Human CDH1 / E Cadherin
Human (tested or 100% immunogen sequence identity)
Tissue culture supernatant
IHC - Paraffin (1:5 - 1:10)
Specificity and Use
CDH1 / E Cadherin antibody was raised against e-cadherin and GST recombinant protein.
Recognizes E-cadherin. Species cross-reactivity: Human.
Suitable for use in Immunohistochemistry. Immunohistochemistry (formalin/paraffin only): (Use Ab at 1:5-1:10 for 30 min at RT) Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Breast carcinoma.
0.09% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
CDH1 / E Cadherin is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis.