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Antibodies > CDH1 / E Cadherin > LS-C407773
 
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Catalog Number Size Price
LS-C407773-10 10 µg $318 
LS-C407773-100 100 µg $470 
CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in frozen section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in frozen section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDH1 / E Cadherin Antibody - E Cadherin antibody IHC-paraffin. IHC(P): Human Placenta Tissue.
CDH1 / E Cadherin Antibody - E Cadherin antibody IHC-frozen. IHC(F): Human Placenta Tissue.
CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDH1 / E Cadherin Antibody - E Cadherin antibody Western blot. All lanes: Anti E Cadherin at 0.5 ug/ml. Lane 1: Human Placenta Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Predicted band size: 140 kD. Observed band size: 140 kD.
CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in frozen section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in frozen section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDH1 / E Cadherin Antibody - E Cadherin antibody IHC-paraffin. IHC(P): Human Placenta Tissue.
CDH1 / E Cadherin Antibody - E Cadherin antibody IHC-frozen. IHC(F): Human Placenta Tissue.
CDH1 / E Cadherin Antibody - IHC analysis of E Cadherin using anti-E Cadherin antibody. E Cadherin was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-E Cadherin Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CDH1 / E Cadherin Antibody - E Cadherin antibody Western blot. All lanes: Anti E Cadherin at 0.5 ug/ml. Lane 1: Human Placenta Tissue Lysate at 50 ug. Lane 2: HELA Whole Cell Lysate at 40 ug. Predicted band size: 140 kD. Observed band size: 140 kD.
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Polyclonal Rabbit anti‑Human CDH1 / E Cadherin Antibody (aa286‑703, IHC, WB) LS‑C407773

Polyclonal Rabbit anti‑Human CDH1 / E Cadherin Antibody (aa286‑703, IHC, WB) LS‑C407773

Submit Review LSBio Guarantee
Antibody:
CDH1 / E Cadherin Rabbit anti-Human Polyclonal (aa286-703) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Human, Rat, Bat, Bovine, Dog, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407773-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CDH1 / E Cadherin Rabbit anti-Human Polyclonal (aa286-703) Antibody
Application:
IHC, IHC-P, IHC-Fr, WB, ELISA
Reactivity:
Human, Rat, Bat, Bovine, Dog, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
E Cadherin antibody LS-C407773 is an unconjugated rabbit polyclonal antibody to E Cadherin (CDH1) (aa286-703) from human. It is reactive with human, rat, bat and other species. Validated for ELISA, IHC and WB.
Target
Human CDH1 / E Cadherin
Synonyms
CDH1 | CD324 | CDHE | Cell-CAM 120/80 | E Cadherin | E-Cadherin | ECAD | Epithelial cadherin | Arc-1 | UVO | Cadherin-1 | CAM 120/80 | CD324 antigen | LCAM | Uvomorulin
Host
Rabbit
Reactivity
Human, Rat, Bat, Bovine, Dog, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human E Cadherin recombinant protein (Position: A286-A703). Human E Cadherin shares 79.7% and 80.9% amino acid (aa) sequence identity with mouse and rat E Cadherin, respectively.
Epitope
aa286-703
Specificity
Non-neural epithelial tissues.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • IHC - Frozen (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDH1 / E Cadherin
P12830 NM_004360 NP_004351.1

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Featured Products

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CDH1 / E Cadherin Antibody LS‑C12333
Species: Human
Applications: IHC, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Functional Assay
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Species: Mouse, Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
CDH1 / E Cadherin Antibody - Anti-E Cadherin antibody IHC of human, spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.
IHC‑plus™ CDH1 / E Cadherin Antibody (clone 3F4) LS‑B4297
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA, Proximity Ligation Assay
CDH1 / E Cadherin Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ CDH1 / E Cadherin Antibody LS‑B12414
Species: Human, Monkey, Mouse, Rat, Dog
Applications: IHC, IHC - Paraffin, Western blot
CDH1 / E Cadherin Antibody - Western blot using CDH1 mouse monoclonal antibody against LNCAP (1),A431 (2), DU145 (3), PC-3 (4), MCF-7 (5), PC-12 (6), NIH/3T3 (7), C6 (8) and COS7 (9) cell lysate.
CDH1 / E Cadherin Antibody (clone 7H12) LS‑C169271
Species: Human
Applications: IHC, Western blot, Flow Cytometry, ELISA
CDH1 / E Cadherin Antibody - Anti-E Cadherin antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC‑plus™ CDH1 / E Cadherin Antibody (aa1‑50) LS‑B5995
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
See all CDH1 / E Cadherin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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