Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
CDCP1 (Cub Domain Containing Protein) was isolated by representational difference analysis using extracts from colon carcinoma and normal colon. It is a putative transmembrane protein with three CUB domains and is thought to be involved in cell adhesion or interaction with the extracellular matrix. CDCP1 undergoes phosphorylation on tyrosine and may undergo shedding as the protein has been detected in the lumen of both normal and cancerous colon crypts by IHC.
Anti-CDCP1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDCP1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDCP1.
Flow cytometry of HeLa cells, using anti-CDCP1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDCP1 antibody, and then analyzed by flow cytometry.
Requested From: United States
Date Requested: 2/26/2017