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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC42 Antibody (aa106‑134) LS‑C168591
CDC42 antibody LS-C168591 is an unconjugated rabbit polyclonal antibody to human CDC42. Validated for Flow and WB.
Catalog
Size
Price
LS-C168591-400
400 µl
Unavailable

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Product Description

CDC42 antibody LS-C168591 is an unconjugated rabbit polyclonal antibody to human CDC42. Validated for Flow and WB.
About CDC42
CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. P60953 NM_001791 NP_001782.1

CDC42 Antibody, G25K Antibody, G25K GTP-binding protein Antibody, Growth-regulating protein Antibody, CDC42Hs Antibody, GTP-binding protein, 25kD Antibody


Specifications

Target
Human CDC42
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Pig, Chicken, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Blocking Peptide
LS-E8558 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C168591. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa106-134
Specificity
This CDC42 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-134 amino acids from the Central region of human CDC42.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of CDC42 Antibody in NCI-H460 cell line lysates (35 ug/lane). CDC42 (arrow) was detected using the purified antibody.
Western blot of CDC42 Antibody in NCI-H460 cell line lysates (35 ug/lane). CDC42 (arrow) was detected using the purified antibody.

Flow Cytometry

CDC42 Antibody flow cytometry of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CDC42 Antibody flow cytometry of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of CDC42 Antibody in NCI-H460 cell line lysates (35 ug/lane). CDC42 (arrow) was detected using the purified antibody.
Western blot of CDC42 Antibody in NCI-H460 cell line lysates (35 ug/lane). CDC42 (arrow) was detected using the purified antibody.

Flow Cytometry

CDC42 Antibody flow cytometry of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CDC42 Antibody flow cytometry of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of CDC42 Antibody in NCI-H460 cell line lysates (35 ug/lane). CDC42 (arrow) was detected using the purified antibody.
Western blot of CDC42 Antibody in NCI-H460 cell line lysates (35 ug/lane). CDC42 (arrow) was detected using the purified antibody.

Flow Cytometry

CDC42 Antibody flow cytometry of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CDC42 Antibody flow cytometry of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of CDC42 Antibody in NCI-H460 cell line lysates (35 ug/lane). CDC42 (arrow) was detected using the purified antibody.
Western blot of CDC42 Antibody in NCI-H460 cell line lysates (35 ug/lane). CDC42 (arrow) was detected using the purified antibody.

Flow Cytometry

CDC42 Antibody flow cytometry of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CDC42 Antibody flow cytometry of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/18/2018

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