Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Locations
Orders Processing,
Shipping & Receiving,
Warehouse
2 Shaker Rd Suites
B001/B101
Shirley, MA 01464
Production Lab
Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036
Telephone Numbers
Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-C18878-100 | 100 µg (1 mg/ml) | $567 |
![CDC27 Antibody - Anti-cdc27 Antibody - Western Blot. Western blot of Affinity Purified anti-cdc27 antibody shows detection of a band ~90 kD corresponding to human cdc27 (arrowhead). Approximately 35 ug of HeLa whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/cdc27-antibody-ls-c18878/62898_51415.jpg)
Polyclonal Rabbit anti‑Human CDC27 Antibody (IF, WB) LS‑C18878
Polyclonal Rabbit anti‑Human CDC27 Antibody (IF, WB) LS‑C18878
Note: This antibody replaces LS-C78933
Antibody:
CDC27 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CDC27 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CDC27 antibody LS-C18878 is an unconjugated rabbit polyclonal antibody to human CDC27. Validated for ELISA, IF and WB.
Target
Human CDC27
Synonyms
CDC27 | ANAPC3 | APC3 | CDC27 homolog | Cell division cycle 27 | D0S1430E | CDC27Hs | D17S978E | H-NUC | Nuc2 homolog | HNUC | NUC2
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Mouse, Rat, Dog, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids 415-445 of Human cdc27.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, chicken, rat and dog based on 100% homology for the immunogen sequence. Expect cross reactivity with cdc27 from mouse as only a single amino acid residue change is found for the immunogen sequence. Cross reactivity with cdc27 homologues from other sources has not been determined.
Applications
- Immunofluorescence
- Western blot (1:500 - 1:2000)
- ELISA (1:5000 - 1:40000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 92 kD in size corresponding to Cdc27 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CDC27
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Reactivity:
Mouse
Range:
31.25-2000 pg/ml
Reactivity:
Human
Range:
0.156-10 ng/ml
Reactivity:
Mouse
Range:
62.5-4000 pg/ml
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, Immunoprecipitation, ELISA
Reactivity:
Human
Range:
40-10000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
