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Anti-CDC25B Antibody (phospho-Ser149) LS-C205318

Catalog Size Price
LS-C205318-50 50 µg (1 mg/ml) Unavailable

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Anti-CDC25B Antibody (aa331-360, Biotin) LS-C254250
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100% Guaranteed
Rabbit Polyclonal to Mouse CDC25B
Mouse, Rat
Western blot
Unconjugated

Details

Mouse CDC25B
Rabbit
Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

Western blot (1:500 - 1:1000)

Specificity and Use

CDC25B antibody was raised against peptide sequence around phosphorylation site of Serine 149 (F-R-S (p) -L-P) of Mouse Cdc25B.
pSer149
The antibody detects endogenous levels of Cdc25B protein only when phosphorylated at serine 149.

Packaging

PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only.

About CDC25B

P30305 NM_021872 NP_068658.1

CDC25B Antibody, CDC25B phosphatase Antibody, Cell division cycle 25B Antibody, M-phase inducer phosphatase 2 Antibody, CDC25HU2 Antibody

CDC25B, a CDC25 dual specificity protein phosphatase, has been shown to affect cell cycle regulation by activating cyclin-dependent kinases Cdk1 and Cdk2 during G2 phase. CDC25B also has been shown to possess oncogenic properties and has been implicated in lung, breast, esophagus, stomach, ovarian, and other cancers. Recently CDC25B has been shown to be a steroid receptor coactivator and this function is independent of the protein phosphatase activity in vitro.

Western blot

Western blot of extracts from 3T3 cells untreated (lane 1) or treated with Hydroxyurea (lane 2) using Cdc25B (Phospho-Ser149) antibody.
Western blot of extracts from 3T3 cells untreated (lane 1) or treated with Hydroxyurea (lane 2) using Cdc25B (Phospho-Ser149) antibody.

Requested From: 
Date Requested: 5/26/2018

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