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Anti-CDC25B Antibody (aa56-105) IHC-plus™ LS-B931

Note: This antibody replaces LS-C30342

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CDC25B Antibodies

Anti-CDC25B Antibody (aa56-105) IHC-plus™ LS-B931
Rabbit Polyclonal to Human CDC25B
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CDC25B Antibody (Internal, Biotin) LS-C254250
Rabbit Polyclonal (IgG) to Human CDC25B
Human
IHC, Western blot, ELISA
Biotin Conjugated
Anti-CDC25B Antibody (phospho-Ser323) LS-C380653
Rabbit Polyclonal (IgG) to Human CDC25B
Human, Mouse, Rat
Western blot, ELISA
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CDC25B
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human CDC25B
Rabbit
Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig (tested or 100% immunogen sequence identity)
Monkey, Bat, Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

CDC25B antibody was raised against synthetic peptide located between aa56-105 of human CDC25B (P30305, NP_068659). Percent identity by BLAST analysis: Human, Gorilla, Galago, Marmoset, Elephant, Panda, Dog, Bovine, Horse, Pig, Guinea pig (100%); Monkey, Bat, Rabbit (92%); Mouse, Rat (85%).
LS-E15204 - Lyophilized - 100 µg - $145.00
aa56-105
Human CDC25B
Immunohistochemistry: LS-B931 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B931 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CDC25B

P30305 NM_021872 NP_068658.1

CDC25B Antibody, CDC25B phosphatase Antibody, Cell division cycle 25B Antibody, M-phase inducer phosphatase 2 Antibody, CDC25HU2 Antibody

CDC25B, a CDC25 dual specificity protein phosphatase, has been shown to affect cell cycle regulation by activating cyclin-dependent kinases Cdk1 and Cdk2 during G2 phase. CDC25B also has been shown to possess oncogenic properties and has been implicated in lung, breast, esophagus, stomach, ovarian, and other cancers. Recently CDC25B has been shown to be a steroid receptor coactivator and this function is independent of the protein phosphatase activity in vitro.

Immunohistochemistry

Immunohistochemistry
Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B931 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
CDC25B antibody LS-B931 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/2/2016

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