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Catalog Number Size Price
LS-B3457-200 200 µl $460 
CDC25A Antibody - Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CDC25A Antibody - Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.
CDC25A Antibody - Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CDC25A Antibody - Western blot of Cdc25A antibody in HeLa cell line lysates (35 ug/lane). Cdc25A (arrow) was detected using the purified antibody.
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IHC‑plus™ Polyclonal Rabbit anti‑Human CDC25A Antibody (aa271‑300, IHC, WB) LS‑B3457

IHC‑plus™ Polyclonal Rabbit anti‑Human CDC25A Antibody (aa271‑300, IHC, WB) LS‑B3457

Note: This antibody replaces LS-C99977
Antibody:
CDC25A Rabbit anti-Human Polyclonal (aa271-300) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B3457-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CDC25A Rabbit anti-Human Polyclonal (aa271-300) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CDC25A antibody LS-B3457 is an unconjugated rabbit polyclonal antibody to human CDC25A (aa271-300). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CDC25A
Synonyms
CDC25A | CDC25A2-CAG isoform | Cell division cycle 25A | CDC25A phosphatase | CDC25Hu1 | M-phase inducer phosphatase 1 | CDC25A2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa271-300
Specificity
This Cdc25A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the Central region of human Cdc25A.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B3457 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3457 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDC25A
P30304 NM_001789 NP_001780.2

Validation

CDC25A Antibody - Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CDC25A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ CDC25A Antibody (aa271-300) for IHC, WB/Western LS-B3457 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/15/2024