Human CD99

Goat

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

NS0-derived rhCD99 extracellular domain.

Extracellular Domain

Selected for its ability to recognize human CD99 in the applications listed. In direct ELISAs and Western Blots, this antibody shows less than 5% cross-reactivity with rmCD99.

• Western blot
• Flow Cytometry
• ELISA

Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody has been validated in whole blood CD3+ T cells. Dilute this antibody to 25 ug/ml and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled antibodies may be visualized by a secondary developing reagent such as donkey anti-goat lgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human CD99. The detection limit for rhCD99 is approximately 0.2 ng/well.

Supplied as a powder lyophilized from a solution containing PBS with 5% trehalose.

Reconstitute with 500uL of sterile PBS.

Store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14209 NCBI: NM_002414 NP_002405.1