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Anti-CD99 Antibody (clone 4A5) LS-C175504

Catalog	Size	Price
LS-C175504-100	100 µl (1 mg/ml)	Unavailable

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Formalin-fixed, paraffin-embedded human Ewing's sarcoma stained with peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells. This image was taken for the unmodified form of this product. Other forms have not been tested.

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Antibody:	Mouse Monoclonal [clone 4A5] (IgG1) to Human CD99
Reactivity:	Human
Application:	IHC - Paraffin, Western blot
Format:	Unconjugated

IHC of paraffin-embedded Human tonsil using anti-CD99 mouse monoclonal antibody.

IHC of paraffin-embedded Human lymphoma tissue using anti-CD99 mouse monoclonal antibody.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD99 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD99.

Details

Target:	Human CD99
Host:	Mouse
Reactivity:	Human (tested or 100% immunogen sequence identity)
Clonality:	IgG1 Monoclonal [4A5]
Conjugations:	Unconjugated
Purification:	Protein A/G purified
Modifications:	Unmodified

Applications

Approved:

IHC - Paraffin (1:150)
Western blot (1:2000)

Specificity and Use

Immunogen:	CD99 antibody was raised against full length human recombinant protein of human CD99 (NP_002405) produced in HEK293T cell.
Specificity:	Human CD99

Packaging

Presentation:	PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Restrictions:	For research use only.

About CD99

Uniprot: P14209 NCBI: NM_002414 NP_002405.1

Synonyms: CD99 Antibody, 12E7 Antibody, CD99 antigen Antibody, MIC2X Antibody, MIC2 Antibody, Surface antigen MIC2 Antibody, MIC2Y Antibody, MSK5X Antibody, Protein MIC2 Antibody, T-cell surface glycoprotein E2 Antibody, CD99 molecule Antibody, E2 antigen Antibody, HBA71 Antibody

Description: CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99.

(More About CD99)

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Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-CD99 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human lymphoma tissue using anti-CD99 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD99 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD99.

Requested From:
Date Requested: 4/19/2018

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