Cat CD95 / FAS

Mouse

Cat (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Phycoerythrin

Protein G purified

Unmodified

NS0-derived recombinant feline rfeFas extracellular domain.

Extracellular Domain

Recognizes feline Fas extracellular domain. Species sequence homology: human 68%, mouse 65%, porcine 53%, and rat 58%. Does not cross-react with rhFas, rmFas, or rrFas.

• Western blot (1 - 2 µg/ml)
• Flow Cytometry (25 µg/ml)
• ELISA (0.5 - 1 µg/ml)

Suitable for use in Flow cytometry, Western blot and Direct ELISA. Flow cytometry: 25 ug/ml and add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: 1-2 ug/ml. the detection limit for rfeFas is approximately 25 ng/lane under non-reducing conditions. It is not recommended to use this antibody under reducing conditions. Chemiluminescent detection with WesternGlo Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. Direct ELISA: 0.5-1.0 ug/ml The detection limit for rfeFas is approximately 3 ng/well. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized PBS, 5% Trehalose

Phosphate Buffered Saline (PBS)

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P25445 NCBI: NM_152877 NP_000034.1