Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse, Bovine (tested or 100% immunogen sequence identity)
Protein G purified
Western blot (0.5 - 2 µg/ml)
Specificity and Use
CD95 / FAS antibody was raised against recombinant protein containing the cytoplasmic domain of mouse Fas.
Recognizes mouse Fas at about 45kD. Species cross-reactivity: bovine.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 ug/ml detects Fas (~45 kD) in RIPA lysates of mouse 3T3/A31 fibroblast cells and bovine brain. Immunoprecipitation: 4 ug immunoprecipitates Fas from 3T3 cells. RIP, a death domain containing protein, was co-precipitated with Fas.
0.1 M Tris-glycine, 0.15 M sodium chloride, pH 7.4, 0.05% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
CD95 / FAS is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10.