Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Tissue culture supernatant
IHC - Paraffin (1:15 - 1:30)
IHC - Frozen (1:15 - 1:30)
Specificity and Use
CD95 / FAS antibody was raised against recombinant protein corresponding to the internal domain near the C-terminal end of the Fas molecule.
Recognizes CD95 (also known as Fas) at 48kD. Species Reactivity: Human.
Suitable for use in Immunohistochemistry. Immunohistochemistry: 1:15-1:30. Frozen & formalin/paraffin. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive control: Small intestine.
0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
CD95 / FAS is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10.