Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone 53-6-7] (IgG2a,k) to Mouse CD8A / CD8 Alpha
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Mouse Monoclonal [clone HIT8a] (IgG1,k) to Human CD8A / CD8 Alpha
Human CD8A / CD8 Alpha
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [HIT8a]
Specificity and Use
The HIT8a antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 ul (0.06 ug)/per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain.
Surface staining of normal human peripheral blood cells with anti-human CD8a (HIT8a) PE. Autofluorescence is indicated by open histogram. Cells in the lymphocyte population were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.