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Anti-CD8A / CD8 Alpha Antibody (clone 17D8, FITC) LS-C140280

Ordering

Wt. Vol. Conc. Price
- 100 tst - $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CD8A / CD8 Alpha Antibodies

Anti-CD8A / CD8 Alpha Antibody (clone 53-6-7) LS-C189666
Rat Monoclonal [clone 53-6-7] (IgG2a,k) to Mouse CD8A / CD8 Alpha
Mouse
IHC - Frozen, Immunoprecipitation, Flow Cytometry
Unconjugated
Anti-CD8A / CD8 Alpha Antibody (C-Terminus, clone C8/144B, Azide-free) LS-C389847
Mouse Monoclonal [clone C8/144B] (IgG1,k) to Human CD8A / CD8 Alpha
Human
IHC - Paraffin, Immunofluorescence, Flow Cytometry
Unconjugated, Azide-free
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 17D8] (IgG1) to Human CD8A / CD8 Alpha
Human
Flow Cytometry
FITC Conjugated

Details

Human CD8A / CD8 Alpha
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [17D8]
Fluorescein
Protein A/G purified
Unmodified

Applications

Flow Cytometry

Specificity and Use

CD8A / CD8 Alpha antibody was raised against human peripheral blood T lymphocytes.
Human CD8A
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Excites at 488nm and emits at 670nm. Store protected from light. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, 0.08% sodium azide, 0.2% carrier protein, sterile-filtered
Store at 4°C. Do not freeze.
For research use only.

About CD8A / CD8 Alpha

P01732 NM_001768 NP_001759.3

CD8A Antibody, CD8a molecule Antibody, CD8a antigen Antibody, OKT8 T-cell antigen Antibody, Leu2 T-lymphocyte antigen Antibody, T8 T-cell antigen Antibody, T cell co-receptor Antibody, p32 Antibody, CD8 Antibody, Leu2 Antibody, T-cell antigen Leu2 Antibody

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain.

Requested From: United States
Date Requested: 12/4/2016

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