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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD86 Antibody (FITC) LS‑C44889
Note: This antibody replaces LS-C57978
Antibody
CD86 antibody LS-C44889 is an FITC-conjugated rat monoclonal antibody to mouse CD86. Validated for Flow.
Catalog
Size
Price
LS-C44889-100
100 µg (0.1 mg/ml)
Unavailable

Popular CD86 Products

Antibody
Species: Human
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: Flow Cytometry
Bovine peripheral blood lymphocytes stained with Mouse anti-Bovine CD86 followed by Goat anti-Mouse IgG:FITC.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Bovine, Sheep
Applications: Flow Cytometry
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

CD86 antibody LS-C44889 is an FITC-conjugated rat monoclonal antibody to mouse CD86. Validated for Flow.
About CD86
CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. It is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. P42081 NM_006889 NP_008820.3

CD86 Antibody, Activation B7-2 antigen Antibody, B7-2 Antibody, B7.2 Antibody, B70 Antibody, CD86 molecule Antibody, CTLA-4 counter-receptor B7.2 Antibody, CD86 antigen Antibody, FUN-1 Antibody, LAB72 Antibody, BU63 Antibody, CD28LG2 Antibody, Ctla-4 counter-receptors b7.2 Antibody


Specifications

Target
Mouse CD86
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Conjugations
Fluorescein. Also available Unconjugated or conjugated with FITC, RPE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Immunogen
CD86 antibody was raised against mouse B-cell line, BCL1.
Specificity
Recognizes mouse CD86 (B7-2), an 80kD cell surface glycoprotein which is a member of the CD28/B7 family. In mouse CD86 is expressed at high levels on peripheral blood monocytes and dendritic cells, and at low levels on resting B and T-lymphocytes. Expression of CD86, on these cell populations, can be increased upon activation. CD86 has been identified, along with CD80 (B7-1), as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA4). CD28 and CTLA4 are two receptors that have opposing functions in T-cell stimulation. Interaction of CD86 with CD28 promotes a number of T-cell activities, whereas the binding of CD86 to CTLA4 inhibits T-cell responses. The PO3 antibody has been reported to block some co-stimulatory functions of CD86. XXX recommend the use of MCA2463EL for this purpose.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Presentation
PBS, pH 7.4, 0.09% sodium azide, 1% BSA.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/18/2018

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