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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD86 Antibody (clone GL1, FITC) LS‑C105985
CD86 antibody LS-C105985 is an FITC-conjugated rat monoclonal antibody to mouse CD86. Validated for Flow.
Catalog
Size
Price
LS-C105985-50
50 µg
Unavailable
LS-C105985-100
100 µg
Unavailable
LS-C105985-500
500 µg
Unavailable

Popular CD86 Products

Antibody
Species: Human
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: Flow Cytometry
Bovine peripheral blood lymphocytes stained with Mouse anti-Bovine CD86 followed by Goat anti-Mouse IgG:FITC.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Bovine, Sheep
Applications: Flow Cytometry
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation

Product Description

CD86 antibody LS-C105985 is an FITC-conjugated rat monoclonal antibody to mouse CD86. Validated for Flow.
About CD86
CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. It is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. P42081 NM_006889 NP_008820.3

CD86 Antibody, Activation B7-2 antigen Antibody, B7-2 Antibody, B7.2 Antibody, B70 Antibody, CD86 molecule Antibody, CTLA-4 counter-receptor B7.2 Antibody, CD86 antigen Antibody, FUN-1 Antibody, LAB72 Antibody, BU63 Antibody, CD28LG2 Antibody, Ctla-4 counter-receptors b7.2 Antibody


Specifications

Target
Mouse CD86
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [GL1]
Conjugations
Fluorescein
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Flow Cytometry
Immunogen
CD86 antibody was raised against mouse CD86
Usage
The GL1 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocyte suspensions. This can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Flow Cytometry

Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Surface staining of LPS stimulated splenocytes with anti-mouse CD86 (GL1) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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