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CD81 Antibody (clone Eat‑2 (EAT2), Biotin) LS‑C106467
CD81 antibody LS-C106467 is a biotin-conjugated hamster monoclonal antibody to mouse CD81. Validated for Flow.
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Species:
Human
Applications:
ICC, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Frozen, Immunofluorescence, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC, Western blot, ELISA
Species:
Human
Applications:
ICC, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Product Description
CD81 antibody LS-C106467 is a biotin-conjugated hamster monoclonal antibody to mouse CD81. Validated for Flow.
About CD81
CD81 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins.
P60033
NM_004356
NP_004347.1
CD81 Antibody, CD81 antigen Antibody, CD81 molecule Antibody, CVID6 Antibody, S5.7 Antibody, TAPA1 Antibody, TAPA-1 Antibody, Tetraspanin-28 Antibody, TSPAN28 Antibody, Tspan-28 Antibody
Specifications
Target
Mouse CD81
Host
Hamster
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG
Monoclonal
[Eat-2 (EAT2)]
Conjugations
Biotin
Purification
Affinity purified
Modifications
Unmodified
Applications
- Flow Cytometry
Immunogen
CD81 antibody was raised against mouse CD81
Usage
The Eat-2 (EAT2) antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Flow Cytometry
Surface staining of mouse splenocytes with 0.25 ug Biotin anti-mouse CD81 (Eat-2) followed by SA-PE. Autofluorescence is shown as open histogram. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow Cytometry
Surface staining of mouse splenocytes with 0.25 ug Biotin anti-mouse CD81 (Eat-2) followed by SA-PE. Autofluorescence is shown as open histogram. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow Cytometry
Surface staining of mouse splenocytes with 0.25 ug Biotin anti-mouse CD81 (Eat-2) followed by SA-PE. Autofluorescence is shown as open histogram. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow Cytometry
Surface staining of mouse splenocytes with 0.25 ug Biotin anti-mouse CD81 (Eat-2) followed by SA-PE. Autofluorescence is shown as open histogram. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/18/2018
Date Requested: 6/18/2018
