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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD80 Antibody (clone RM80, RPE) LS‑C57975
Note: This antibody replaces LS-C57976
Antibody
CD80 antibody LS-C57975 is an RPE-conjugated rat monoclonal antibody to mouse CD80. Validated for Flow and IHC.
Catalog
Size
Price
LS-C57975-100
100 tst
Unavailable

Popular CD80 Products

Antibody
Species: Bovine, Human
Applications: Western blot, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: Flow Cytometry
Anti-CD80 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4109 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CD80 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
IHC of paraffin-embedded Human tonsil using anti-CD80 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

CD80 antibody LS-C57975 is an RPE-conjugated rat monoclonal antibody to mouse CD80. Validated for Flow and IHC.
About CD80
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. P33681 NM_005191 NP_005182.1

CD80 Antibody, Activation B7-1 antigen Antibody, B7-1 Antibody, B7.1 Antibody, BB1 Antibody, CD28LG Antibody, CD28LG1 Antibody, CD80 antigen Antibody, LAB7 Antibody, CD80 molecule Antibody, CTLA-4 counter-receptor B7.1 Antibody


Specifications

Target
Mouse CD80
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [RM80]
Conjugations
R. Phycoerythrin. Also available Unconjugated or conjugated with FITC.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Flow Cytometry (1:1)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD80 antibody was raised against bCL1 cells expressing CD80.
Specificity
Recognizes mouse CD80 (B7-1), a 60kD cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4. CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation. The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 1x10^6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Presentation
Lyophilized, PBS, pH 7.4, 0.09% sodium azide, 1% BSA.
Reconstitution
100 tst Distilled Water.
Storage
4°C, avoid freezing.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/19/2018

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